|
週次 |
日期 |
單元主題 |
|
第1週 |
09/12 |
Introduction and biology of culture cells
Equipment and aseptic technique.
Assign teams and culture room guide
Microscope, Laminar flow hood, Incubator, Washing and sterilizing glassware. |
|
第2週 |
09/19 |
Medium and supplements
Culture vessels and substrates.
Search cell line information form ATCC website. |
|
第3週 |
09/26 |
Transformation and Immortalization |
|
第4週 |
10/03 |
Cell growth curve and primary culture |
|
第5週 |
10/10 |
Double 10 Day (放假) |
|
第6週 |
10/17 |
Cell contamination and characterization |
|
第7週 |
10/24 |
Stem cells |
|
第8週 |
10/31 |
Characterization of mesenchymal |
|
第9週 |
11/07 |
Mid-term examination |
|
第10週 |
11/14 |
Thaw cell (NIH3T3) from liquid nitrogen, counting cells by hemocytometer, plating cells and maintenance
Feeding a monolayer culture, trypsinization, cell count and seeding the cells |
|
第11週 |
11/21 |
Cell line culture, subculture and Quantitation, maintenance growth curve measurement.
Embryonic fibroblast primary culture, maintenance growth curve measurement |
|
第12週 |
11/28 |
Cryopreservation of cultured cells |
|
第13週 |
12/05 |
Embryonic primary fibroblast culture |
|
第14週 |
12/12 |
Embryonic primary fibroblast culture |
|
第15週 |
12/19 |
Mesenchymal stem cells culture |
|
第16週 |
12/26 |
Mesenchymal stem cells culture and induction of differentiation |
|
第17週 |
01/02 |
Epithelial culture and characterization |
|
第18週 |
01/09 |
Final Report |